Metabolic physiology and skeletal muscle phenotypes in male and female myoglobin knockout mice.

ABSTRACT: Myoglobin (Mb) is a regulator of O₂ bioavailability in type I muscle and heart, at least when tissue O₂ levels drop. Mb also plays a role in regulating cellular nitric oxide (NO) pools. Robust binding of long-chain fatty acids and long-chain acylcarnitines to Mb, and enhanced glucose metabolism in hearts of Mb knockout (KO) mice, suggest additional roles in muscle intermediary metabolism and fuel selection. To evaluate this hypothesis, we measured energy expenditure (EE), respiratory exchange ratio (RER), body weight gain and adiposity, glucose tolerance, and insulin sensitivity in Mb knockout (Mb^-/-) and wild-type (WT) mice challenged with a high-fat diet (HFD, 45% of calories). In males (n = 10/genotype) and females (n = 9/genotype) tested a

SUBMITTER: Ono-Moore KD 

PROVIDER: S-EPMC8321820 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature