Expression of serum response factor in the lung mesenchyme is essential for development of pulmonary fibrosis.

ABSTRACT: Extracellular matrix deposition characterizes idiopathic pulmonary fibrosis (IPF) and is orchestrated by myofibroblasts. The lung mesenchyme is an essential source of myofibroblasts in pulmonary fibrosis. Although the transcription factor serum response factor (SRF) has shown to be critical in the process of myofibroblast differentiation, its role in development of pulmonary fibrosis has not been determined in vivo. In this study, we observed that SRF expression localized to mesenchymal compartments, areas of dense fibrosis, and fibroblastic foci in human (IPF and normal) and bleomycin-treated mouse lungs. To determine the role of mesenchymal SRF in pulmonary fibrosis, we utilized a doxycycline-inducible, Tbx4 lung enhancer (Tbx4^LE)-driven Cre-recombinase to disrupt SRF e

SUBMITTER: Bernau K 

PROVIDER: S-EPMC8321854 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature