Morpho-metabotyping the oxidative stress response.

ABSTRACT: Oxidative stress and reactive oxygen species (ROS) are central to many physiological and pathophysiological processes. However, due to multiple technical challenges, it is hard to capture a comprehensive readout of the cell, involving both biochemical and functional status. We addressed this problem by developing a fully parallelized workflow for metabolomics (providing absolute quantities for > 100 metabolites including TCA cycle, pentose phosphate pathway, purine metabolism, glutathione metabolism, cysteine and methionine metabolism, glycolysis and gluconeogenesis) and live cell imaging microscopy. The correlative imaging strategy was applied to study morphological and metabolic adaptation of cancer cells upon short-term hydrogen peroxide (H₂O₂) exposure in vitro. T

SUBMITTER: Rusz M 

PROVIDER: S-EPMC8322264 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature