Anisotropy in mechanical unfolding of protein upon partner-assisted pulling and handle-assisted pulling.

ABSTRACT: Proteins as force-sensors respond to mechanical cues and regulate signaling in physiology. Proteins commonly connect the source and response points of mechanical cues in two conformations, independent proteins in end-to-end geometry and protein complexes in handshake geometry. The force-responsive property of independent proteins in end-to-end geometry is studied extensively using single-molecule force spectroscopy (SMFS). The physiological significance of the complex conformations in force-sensing is often disregarded as mere surge protectors. However, with the potential of force-steering, protein complexes possess a distinct mechano-responsive property over individual force-sensors. To decipher, we choose a force-sensing protein, cadherin-23, from tip-link complex and perform SMFS using

SUBMITTER: Arora N 

PROVIDER: S-EPMC8322310 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature