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Proteomic study of Desulfovibrio ferrophilus IS5 reveals overexpressed extracellular multi-heme cytochrome associated with severe microbiologically influenced corrosion.

ABSTRACT: Microbiologically influenced corrosion (MIC) is recognized as a considerable threat to carbon steel asset integrity in the oil and gas industry. There is an immediate need for reliable and broadly applicable methods for detection and monitoring of MIC. Proteins associated with microbial metabolisms involved in MIC could serve as useful biomarkers for MIC diagnosis and monitoring. A proteomic study was conducted using a lithotrophically-grown bacterium Desulfovibrio ferrophilus strain IS5, which is known to cause severe MIC in seawater environments. Unique proteins, which are differentially and uniquely expressed during severe microbial corrosion by strain IS5, were identified. This includes the detection of a multi-heme cytochrome protein possibly involved in extracellular electron transfer in the presence of the carbon steel. Thus, we conclude that this newly identified protein associated closely with severe MIC could be used to generate easy-to-implement immunoassays for reliable detection of microbiological corrosion in the field.

SUBMITTER: Chatterjee M

PROVIDER: S-EPMC8322314 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Proteomic study of Desulfovibrio ferrophilus IS5 reveals overexpressed extracellular multi-heme cytochrome associated with severe microbiologically influenced corrosion.

Chatterjee Mohor M Fan Yu Y Cao Fang F Jones Aaron A AA Pilloni Giovanni G Zhang Xiaozhou X

Scientific reports 20210729 1

Microbiologically influenced corrosion (MIC) is recognized as a considerable threat to carbon steel asset integrity in the oil and gas industry. There is an immediate need for reliable and broadly applicable methods for detection and monitoring of MIC. Proteins associated with microbial metabolisms involved in MIC could serve as useful biomarkers for MIC diagnosis and monitoring. A proteomic study was conducted using a lithotrophically-grown bacterium Desulfovibrio ferrophilus strain IS5, which ...[more]

PMID: 34326431

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Project description:Microbiologically Influenced Corrosion (MIC) poses a significant challenge to various industries, leading to substantial economic losses and potential safety hazards. Despite extensive research on the MIC resistance of various materials, there is a lack of studies focusing on High Chromium White Iron (HCWI) alloys, which are widely used in wear-resistant applications. This study addresses this knowledge gap by providing a comprehensive investigation of the MIC resistance of three HCWI alloys with varying chromium contents (22 wt%, 30.7 wt%, and 21 wt%) in the presence of Pseudomonas aeruginosa (P. Aeruginosa), a common bacterial species associated with MIC. The alloys were exposed to an artificial seawater medium inoculated with P.Aeruginosa for 14 days, and their corrosion behaviour was evaluated using electrochemical techniques, surface analysis, and microscopy. Electrochemical Impedance Spectroscopy (EIS) results revealed that the alloy with the highest chromium content (A2, 30.7 wt% Cr) exhibited superior MIC resistance compared to the other alloys (A1, 22 wt% Cr and M1, 21 wt% Cr). The enhanced performance of alloy A2 was attributed to the formation of a more stable and protective passive film, as well as the development of a more compact and less permeable biofilm. The EIS data, interpreted using equivalent circuit models, showed that alloy A2 had the highest charge transfer resistance and the lowest biofilm capacitance, indicating a more effective barrier against corrosive species. Bode plots further confirmed the superior corrosion resistance of alloy A2, with higher impedance values and phase angles at low frequencies compared to alloys A1 and M1. Scanning Electron Microscopy (SEM) and optical microscopy analyses corroborated these findings, showing that alloy A2 had the lowest pit density and size after 14 days of exposure. The insights gained from this study highlight the critical role of chromium content in the MIC resistance of HCWI alloys and have significant implications for the design and selection of materials for applications prone to microbial corrosion.

Dataset Information

Proteomic study of Desulfovibrio ferrophilus IS5 reveals overexpressed extracellular multi-heme cytochrome associated with severe microbiologically influenced corrosion.

Publications

Proteomic study of Desulfovibrio ferrophilus IS5 reveals overexpressed extracellular multi-heme cytochrome associated with severe microbiologically influenced corrosion.

Similar Datasets

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