Novel rRNA-depletion methods for total RNA sequencing and ribosome profiling developed for avian species.

ABSTRACT: Deep sequencing of RNAs has greatly aided the study of the transcriptome, enabling comprehensive gene expression profiling and the identification of novel transcripts. While messenger RNAs (mRNAs) are of the greatest interest in gene expression studies as they encode for proteins, mRNAs make up only 3 to 5% of total RNAs, with the majority comprising ribosomal RNAs (rRNAs). Therefore, applications of deep sequencing to RNA face the challenge of how to efficiently enrich mRNA species prior to library construction. Traditional methods extract mRNAs using oligo-dT primers targeting the poly-A tail on mRNAs; however, this approach is not comprehensive as it does not capture mRNAs lacking the poly-A tail or other long non-coding RNAs that we may be interested in. Alternative mRNA enrichment met

SUBMITTER: Gu H 

PROVIDER: S-EPMC8322463 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature