Construction of T7-Like Expression System in Pseudomonas putida KT2440 to Enhance the Heterologous Expression Level.

ABSTRACT: Pseudomonas putida KT2440 has become an attractive chassis for heterologous expression with the development of effective genetic manipulation tools. Improving the level of transcriptional regulation is particularly important for extending the potential of P. putida KT2440 in heterologous expression. Although many strategies have been applied to enhance the heterologous expression level in P. putida KT2440, it was still at a relatively low level. Herein we constructed a T7-like expression system in P. putida KT2440, mimicking the pET expression system in Escherichia coli, which consisted of T7-like RNA polymerase (MmP1) integrated strain and the corresponding expression vector for the heterologous expression enhancement. With the optimization of the insert

SUBMITTER: Liang T 

PROVIDER: S-EPMC8322953 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature