Creation of a Single Cell RNASeq Meta-Atlas to Define Human Liver Immune Homeostasis.

ABSTRACT: The liver is unique in both its ability to maintain immune homeostasis and in its potential for immune tolerance following solid organ transplantation. Single-cell RNA sequencing (scRNA seq) is a powerful approach to generate highly dimensional transcriptome data to understand cellular phenotypes. However, when scRNA data is produced by different groups, with different data models, different standards, and samples processed in different ways, it can be challenging to draw meaningful conclusions from the aggregated data. The goal of this study was to establish a method to combine 'human liver' scRNA seq datasets by 1) characterizing the heterogeneity between studies and 2) using the meta-atlas to define the dominant phenotypes across immune cell subpopulations in healthy human liver. Public

SUBMITTER: Rocque B 

PROVIDER: S-EPMC8322955 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature