A comprehensive approach for microbiota and health monitoring in mouse colonies using metagenomic shotgun sequencing.

ABSTRACT:

Background

Health surveillance of murine colonies employed for scientific purposes aim at detecting unwanted infection that can affect the well-being of animals and personnel, and potentially undermine scientific results. In this study, we investigated the use of a next-generation sequencing (NGS) metagenomic approach for monitoring the microbiota composition and uncovering the possible presence of pathogens in mice housed in specific pathogen-free (SPF) or conventional (non-SPF) facilities.

Results

Analysis of metagenomic NGS assay through public and free algorithms and databases allowed to precisely assess the composition of mouse gut microbiome and quantify the contribution of the different microorganisms at the species level. Sequence analysis allowed the uncovering of p

SUBMITTER: Scavizzi F 

PROVIDER: S-EPMC8323313 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature