Cross-Link-Functionalized Nanoparticles for Rapid Excretion in Nanotheranostic Applications.

ABSTRACT: Most NIR-IIb fluorophores are nanoparticle-based probes with long retention ( ≈ 1 month or longer) in the body. Here, we applied a novel cross-linked coating to functionalize core/shell lead sulfide/cadmium sulfide quantum dots (PbS/CdS QDs) emitting at ≈ 1600 nm. The coating was comprised of an amphiphilic polymer followed by three crosslinked amphiphilic polymeric layers (P³ coating), imparting high biocompatibility and > 90% excretion of QDs within 2 weeks of intravenous administration. The P³-QDs were conjugated to an engineered anti-CD8 diabody (Cys-diabody) for in vivo molecular imaging of CD8 + cytotoxic T lymphocytes (CTLs) in response to anti-PD-L1 therapy. Two-plex molecular imaging in combination with down-conversion Er nanoparticles (ErNPs) was performed f

SUBMITTER: Ma Z 

PROVIDER: S-EPMC8324002 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature