Single-cell analyses unravel cell type-specific responses to a vitamin D analog in prostatic precancerous lesions.

ABSTRACT: Epidemiological data have linked vitamin D deficiency to the onset and severity of various cancers, including prostate cancer, and although in vitro studies have demonstrated anticancer activities for vitamin D, clinical trials provided conflicting results. To determine the impact of vitamin D signaling on prostatic precancerous lesions, we treated genetically engineered Pten(i)pe-/- mice harboring prostatic intraepithelial neoplasia (PIN) with Gemini-72, a vitamin D analog with reported anticancer activities. We show that this analog induces apoptosis in senescent PINs, normalizes extracellular matrix remodeling by stromal fibroblasts, and reduces the prostatic infiltration of immunosuppressive myeloid-derived suppressor cells. Moreover, single-cell RNA-sequencing analysis demonstrates th

SUBMITTER: Abu El Maaty MA 

PROVIDER: S-EPMC8324049 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature