Pulsed Third-Spin-Assisted Recoupling NMR for Obtaining Long-Range ¹³C-¹³C and ¹⁵N-¹³C Distance Restraints.

ABSTRACT: We present a class of pulsed third-spin-assisted recoupling (P-TSAR) magic-angle-spinning solid-state NMR techniques that achieve efficient polarization transfer over long distances to provide important restraints for structure determination. These experiments utilize second-order cross terms between strong ¹H-¹³C and ¹H-¹⁵N dipolar couplings to achieve ¹³C-¹³C and ¹⁵N-¹³C polarization transfer, similar to the principle of continuous-wave (CW) TSAR experiments. However, in contrast to the CW-TSAR experiments, these P-TSAR experiments require much less radiofrequency (rf) energy and allow a much simpler routine for optimizing the rf field strength. We call the technique PULSAR (pulsed proton-assisted rec

SUBMITTER: Gelenter MD 

PROVIDER: S-EPMC8324326 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature