Quantification of creatine kinase reaction rate in mouse hindlimb using phosphorus-31 magnetic resonance spectroscopic fingerprinting.

ABSTRACT: The goal of this study was to evaluate the accuracy, reproducibility, and efficiency of a ³¹ P magnetic resonance spectroscopic fingerprinting (³¹ P-MRSF) method for fast quantification of the forward rate constant of creatine kinase (CK) in mouse hindlimb. The ³¹ P-MRSF method acquired spectroscopic fingerprints using interleaved acquisition of phosphocreatine (PCr) and γATP with ramped flip angles and a saturation scheme sensitive to chemical exchange between PCr and γATP. Parameter estimation was performed by matching the acquired fingerprints to a dictionary of simulated fingerprints generated from the Bloch-McConnell model. The accuracy of ³¹ P-MRSF measurements was compared with the magnetization transfer (MT-MRS) method in mouse hindlimb a

SUBMITTER: Kim K 

PROVIDER: S-EPMC8324327 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature