Migration speed of nucleolus precursor bodies in human male pronuclei: a novel parameter for predicting live birth.

ABSTRACT:

Purpose

To study the relationship between the migration speed of nucleolus precursor bodies (NPBs) in male and female pronuclei (mPN; fPN) and human embryo development during assisted reproduction.

Methods

The migration speed of 263 NPBs from 47 zygotes was quantitated, and embryonic development was observed until the blastocyst stage. The central coordinates of mPN, fPN, and NPBs were noted at multiple timepoints. Then, the distance traveled by the NPBs between two sequential images was measured, and migration speed was calculated. Additionally, we investigated the relationship between NPB migration speed and ploidy status (N = 33) or live birth/ongoing pregnancy (LB/OP) (N = 60) after assisted reproduction.

Results

The NPB migration speed in both mPN and fPN was sig

SUBMITTER: Inoue T 

PROVIDER: S-EPMC8324659 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature