Dataset Information

Association of a monopartite begomovirus and associated betasatellite with yellow vein disease of a weed host, Senna italica Mill. In Oman.

ABSTRACT: Senna italica Mill. plants exhibiting yellowing and stunting symptoms typical of begomovirus infection were collected in Oman. Molecular characterization using begomovirus and betasatellite primers in polymerase chain reaction followed by rolling circle amplification, cloning and analysis of sequences revealed the S. italica plants were infected by an isolate of Chilli leaf curl virus and tomato leaf curl betasatellite. The study describes the etiology of a yellow vein disease, identified for the first time, affecting a common weed in Oman.

SUBMITTER: Al-Mabsli SS

PROVIDER: S-EPMC8324710 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Association of a monopartite begomovirus and associated betasatellite with yellow vein disease of a weed host, Senna italica Mill. In Oman.

Al-Mabsli Salma Saif SS Al-Wahaibi Ali K AK Al-Sadi A M AM Shahid M S MS

Virusdisease 20210428 2

Senna italica Mill. plants exhibiting yellowing and stunting symptoms typical of begomovirus infection were collected in Oman. Molecular characterization using begomovirus and betasatellite primers in polymerase chain reaction followed by rolling circle amplification, cloning and analysis of sequences revealed the S. italica plants were infected by an isolate of Chilli leaf curl virus and tomato leaf curl betasatellite. The study describes the etiology of a yellow vein disease, ide ...[more]

PMID: 34350321

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Project description:Mentha is a very important crop grown and used extensively for many purposes in the Kingdom of Saudi Arabia. Begomoviruses are whitefly-transmitted viruses causing serious disease in many important plants exhibiting variable symptoms with significant economic loss globally. During farmers' field survey, yellow vein mosaic disease was observed in Mentha longifolia plants growing near tomato fields in Saudi Arabia. The causative agent was identified in 11 out of 19 samples using begomovirus-specific primers and the association of begomovirus with yellow vein mosaic disease in M. longifolia was confirmed. The full-length viral genome and betasatellite were amplified, cloned, and sequenced bidirectionally. The full DNA-A genome was found to have 2785 nucleotides with 1365 bp-associated betasatellite molecule. An attempt was made to amplify DNA-B, but none of the samples produced any positive amplicon of expected size which indicated the presence of monopartite begomovirus. The sequence identity matrix and phylogenetic analysis, based on full genome showed the highest identity (99.6%) with Tomato yellow leaf curl virus (TYLCV) and in phylogenetic analysis it formed a closed cluster with Tomato leaf curl virus infecting tomato and Corchorus crop in Saudi Arabia. The sequence analysis results of betasatellites showed the highest identity (98.9%) with Tomato yellow leaf curl betasatellites infecting tomato and phylogenetic analysis using betasatellites formed a close cluster with Tomato yellow leaf curl betasatellites infecting tomato and Corchorus crops, which has already been reported to cause yellow vein mosaic and leaf curl disease in many cultivated and weed crops growing in Saudi Arabia. The identified begomovirus associated with yellow vein mosaic disease in mentha could be a mutated strain of TYLCV and tentatively designated as TYLCV-Mentha isolate. Based on published data and latest information, this is the first report of identification of Tomato yellow leaf curl virus associated with yellow vein mosaic disease of M. longifolia from Saudi Arabia.

Project description:BackgroundTomato yellow leaf curl virus (TYLCV), a monopartite begomovirus (family Geminiviridae) is responsible for heavy yield losses for tomato production around the globe. In Oman at least five distinct begomoviruses cause disease in tomato, including TYLCV. Unusually, TYLCV infections in Oman are sometimes associated with a betasatellite (Tomato leaf curl betasatellite [ToLCB]; a symptom modulating satellite). RNA interference (RNAi) can be used to develop resistance against begomoviruses at either the transcriptional or post-transcriptional levels.ResultsA hairpin RNAi (hpRNAi) construct to express double-stranded RNA homologous to sequences of the intergenic region, coat protein gene, V2 gene and replication-associated gene of Tomato yellow leaf curl virus-Oman (TYLCV-OM) was produced. Initially, transient expression of the hpRNAi construct at the site of virus inoculation was shown to reduce the number of plants developing symptoms when inoculated with either TYLCV-OM or TYLCV-OM with ToLCB-OM to Nicotiana benthamiana or tomato. Solanum lycopersicum L. cv. Pusa Ruby was transformed with the hpRNAi construct and nine confirmed transgenic lines were obtained and challenged with TYLCV-OM and ToLCB-OM by Agrobacterium-mediated inoculation. For all but one line, for which all plants remained symptomless, inoculation with TYLCV-OM led to a proportion (≤25%) of tomato plants developing symptoms of infection. For inoculation with TYLCV-OM and ToLCB-OM all lines showed a proportion of plants (≤45%) symptomatic. However, for all infected transgenic plants the symptoms were milder and virus titre in plants was lower than in infected non-transgenic tomato plants.ConclusionsThese results show that RNAi can be used to develop resistance against geminiviruses in tomato. The resistance in this case is not immunity but does reduce the severity of infections and virus titer. Also, the betasatellite may compromise resistance, increasing the proportion of plants which ultimately show symptoms.

Dataset Information

Association of a monopartite begomovirus and associated betasatellite with yellow vein disease of a weed host, Senna italica Mill. In Oman.

Publications

Association of a monopartite begomovirus and associated betasatellite with yellow vein disease of a weed host, <i>Senna italica</i> Mill. In Oman.

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