Forecasting early onset diminished ovarian reserve for young reproductive age women.

ABSTRACT:

Purpose

To investigate the biological networks associated with DOR in young women and the subsequent molecular impact on preimplantation embryos.

Methods

Whole peripheral blood was collected from patients: young women presenting with diminished ovarian reserve (DOR) and age-matched young women with normal ovarian reserve. Maternal exome sequencing was performed on the NovaSEQ 6000 and sequencing validation was completed using Taqman® SNP Genotyping Assays. Blastocyst global methylome and transcriptome sequencing were also analyzed.

Results

Exome sequencing revealed 730 significant DNA variants observed exclusively in the young DOR patients. Bioinformatic analysis revealed a significant impact to the Glucocorticoid receptor (GR) signaling pathway and each young DOR fem

SUBMITTER: McCallie BR 

PROVIDER: S-EPMC8324723 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature