Embryo morphokinetic score is associated with biomarkers of developmental competence and implantation.

ABSTRACT:

Purpose

To study embryo morphokinetics in relation to release in spent media of molecules with possible roles in development and implantation (miR-20a, miR-30c, and sHLA-G).

Methods

Data were obtained from embryos generated in standard IVF and ICSI cycles. The Eeva system was used for embryo assessment, based on early morphokinetic parameters and producing a score (1-5, best-worst) corresponding to higher/medium/lower chances of development to blastocyst. miRNAs - mm miR-20a-5p and miR-30c-5p - and sHLA-G were quantified in 25 μl of spent blastocyst media (SBM) collected before vitrification or transfer. Statistical analyses were performed applying Kolmogorov-Smirnov, Shapiro-Wilk, and Spearman's correlation coefficient tests, where appropriate.

Results

SBM were colle

SUBMITTER: Coticchio G 

PROVIDER: S-EPMC8324755 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature