Production and characterization of a human antisperm monoclonal antibody against CD52g for topical contraception in women.

ABSTRACT:

Background

Approximately 40% of human pregnancies are unintended, indicating a need for more acceptable effective contraception methods. New antibody production systems make it possible to manufacture reagent-grade human monoclonal antibodies (mAbs) for clinical use. We used the Nicotiana platform to produce a human antisperm mAb and tested its efficacy for on-demand topical contraception.

Methods

Heavy and light chain variable region DNA sequences of a human IgM antisperm antibody derived from an infertile woman were inserted with human IgG₁ constant region sequences into an agrobacterium and transfected into Nicotiana benthamiana. The product, an IgG₁ mAb ["Human Contraception Antibody" (HCA)], was purified on Protein A columns, and QC was performed u

SUBMITTER: Baldeon-Vaca G 

PROVIDER: S-EPMC8324805 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature