Subcellular localization of biomolecules and drug distribution by high-definition ion beam imaging.

ABSTRACT: Simultaneous visualization of the relationship between multiple biomolecules and their ligands or small molecules at the nanometer scale in cells will enable greater understanding of how biological processes operate. We present here high-definition multiplex ion beam imaging (HD-MIBI), a secondary ion mass spectrometry approach capable of high-parameter imaging in 3D of targeted biological entities and exogenously added structurally-unmodified small molecules. With this technology, the atomic constituents of the biomolecules themselves can be used in our system as the "tag" and we demonstrate measurements down to ~30 nm lateral resolution. We correlated the subcellular localization of the chemotherapy drug cisplatin simultaneously with five subnuclear structures. Cisplatin was preferential

SUBMITTER: Rovira-Clave X 

PROVIDER: S-EPMC8324837 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature