Integration of sample preparation with RNA-Amplification in a hand-held device for airborne virus detection.

ABSTRACT: Aerosol transmission is one of the three major transmission routes of respiratory viruses. However, the dynamics and significance of the aerosol transmission route are not well understood, partially due to the lack of rapid and efficient tools for on-the-spot detection of airborne viruses. We report a hand-held device that integrates a 3D-printed sample preparation unit with a laminated paper-based RNA amplification unit. The sample preparation unit features an innovative reagent delivery scheme based on a ball-based valve capable of storing and delivering reagents through the rotation of the unit without manual pipetting, while the paper-based unit enables RNA enrichment and reverse transcription loop-mediated isothermal amplification (RT-LAMP). We have determined the detection limit of t

SUBMITTER: Jiang X 

PROVIDER: S-EPMC8325109 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature