Temperature effects on RNA polymerase initiation kinetics reveal which open complex initiates and that bubble collapse is stepwise.

ABSTRACT: Transcription initiation is highly regulated by promoter sequence, transcription factors, and ligands. All known transcription inhibitors, an important class of antibiotics, act in initiation. To understand regulation and inhibition, the biophysical mechanisms of formation and stabilization of the "open" promoter complex (OC), of synthesis of a short RNA-DNA hybrid upon nucleotide addition, and of escape of RNA polymerase (RNAP) from the promoter must be understood. We previously found that RNAP forms three different OC with λP_R promoter DNA. The 37 °C RNAP-λP_R OC (RP_O) is very stable. At lower temperatures, RP_O is less stable and in equilibrium with an intermediate OC (I₃). Here, we report step-by-step rapid quench-flow kinetic data f

SUBMITTER: Plaskon DM 

PROVIDER: S-EPMC8325161 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature