Molecular features and vulnerabilities of recurrent chordomas.

ABSTRACT:

Background

Tumor recurrence is one of the major challenges in clinical management of chordoma. Despite R0-resection, approximately 50% of chordomas recur within ten years after initial surgery. The underlying molecular processes are poorly understood resulting in the lack of associated therapeutic options. This is not least due to the absence of appropriate cell culture models of this orphan disease.

Methods

The intra-personal progression model cell lines U-CH11 and U-CH11R were compared using array comparative genomic hybridization, expression arrays, RNA-seq, and immunocytochemistry. Cell line origin was confirmed by short tandem repeat analysis. Inter-personal cell culture models (n = 6) were examined to validate whether the new model is representative. Cell viability aft

SUBMITTER: Seeling C 

PROVIDER: S-EPMC8325178 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature