Assessing the antigenicity of different VP3 regions of infectious bursal disease virus in chickens from South Brazil.

ABSTRACT:

Background

Infectious bursal disease (IBD), also known as Gumboro disease, is a viral infection that causes mortality and immunosuppression in chickens (Gallus gallus). VP2 and VP3 are the major structural viral capsid components and are the most immunogenic proteins of IBD virus (IBDV). Reliable diagnostic tests using VP2 and VP3 produced in heterologous systems are important tools to control this infection. One advantage of an IBD diagnostic based on VP3, over those that use VP2, is that VP3 has linear epitopes, enabling its production in bacteria.

Results

We tested the suitability of recombinant VP3 (rVP3) as a diagnostic reagent in an enzyme-linked immunosorbent assay (ELISA). Compared with a commercial test, rVP3 ELISA showed high sensitivity and specificity as a diagno

SUBMITTER: Palka APG 

PROVIDER: S-EPMC8325195 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature