Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion.

ABSTRACT: Biofilms that form on implanted medical devices cause recalcitrant infections. The early events enabling contaminating bacteria to evade immune clearance, before a mature biofilm is established, are poorly understood. Live imaging in vitro demonstrated that Staphylococcus aureus sparsely inoculated on an abiotic surface can go undiscovered by human neutrophils, grow, and form aggregates. Small (~50 μm²) aggregates of attached bacteria resisted killing by human neutrophils, resulting in neutrophil lysis and bacterial persistence. In vivo, neutrophil recruitment to a peritoneal implant was spatially heterogenous, with some bacterial aggregates remaining undiscovered by neutrophils after 24 h. Intravital imaging in mouse skin revealed that attached S. aureus aggregates grew and rem

SUBMITTER: Pettygrove BA 

PROVIDER: S-EPMC8325624 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature