Twinfilin1 controls lamellipodial protrusive activity and actin turnover during vertebrate gastrulation.

ABSTRACT: The dynamic control of the actin cytoskeleton is a key aspect of essentially all animal cell movements. Experiments in single migrating cells and in vitro systems have provided an exceptionally deep understanding of actin dynamics. However, we still know relatively little of how these systems are tuned in cell-type-specific ways, for example in the context of collective cell movements that sculpt the early embryo. Here, we provide an analysis of the actin-severing and depolymerization machinery during vertebrate gastrulation, with a focus on Twinfilin1 (Twf1) in Xenopus. We find that Twf1 is essential for convergent extension, and loss of Twf1 results in a disruption of lamellipodial dynamics and polarity. Moreover, Twf1 loss results in a failure to assemble polarized cytoplasmic actin cab

SUBMITTER: Devitt CC 

PROVIDER: S-EPMC8325956 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature