Characterization of the Impact of Density Gradient Centrifugation on the Profile of the Pig Sperm Transcriptome by RNA-Seq.

ABSTRACT: RNA-Seq data from human semen suggests that the study of the sperm transcriptome requires the previous elimination from the ejaculates of somatic cells carrying a larger load of RNA. Semen purification is also carried to study the sperm transcriptome in other species including swine and it is often done by density gradient centrifugation to obtain viable spermatozoa from fresh ejaculates or artificial insemination doses, thereby limiting the throughput and remoteness of the samples that can be processed in one study. The aim of this work was to evaluate the impact of purification with density gradient centrifugation by BoviPure^TM on porcine sperm. Four boar ejaculates were purified with BoviPure^TM and their transcriptome sequenced by RNA-Seq was compared with the RNA-

SUBMITTER: Lian Y 

PROVIDER: S-EPMC8326511 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature