Microfluidic-based imaging of complete Caenorhabditis elegans larval development.

ABSTRACT: Several microfluidic-based methods for Caenorhabditis elegans imaging have recently been introduced. Existing methods either permit imaging across multiple larval stages without maintaining a stable worm orientation, or allow for very good immobilization but are only suitable for shorter experiments. Here, we present a novel microfluidic imaging method that allows parallel live-imaging across multiple larval stages, while maintaining worm orientation and identity over time. This is achieved through an array of microfluidic trap channels carefully tuned to maintain worms in a stable orientation, while allowing growth and molting to occur. Immobilization is supported by an active hydraulic valve, which presses worms onto the cover glass during image acquisition only. In this way, excellent q

SUBMITTER: Berger S 

PROVIDER: S-EPMC8327290 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature