Long-read RNA sequencing reveals widespread sex-specific alternative splicing in threespine stickleback fish.

ABSTRACT: Alternate isoforms are important contributors to phenotypic diversity across eukaryotes. Although short-read RNA-sequencing has increased our understanding of isoform diversity, it is challenging to accurately detect full-length transcripts, preventing the identification of many alternate isoforms. Long-read sequencing technologies have made it possible to sequence full-length alternative transcripts, accurately characterizing alternative splicing events, alternate transcription start and end sites, and differences in UTR regions. Here, we use Pacific Biosciences (PacBio) long-read RNA-sequencing (Iso-Seq) to examine the transcriptomes of five organs in threespine stickleback fish (Gasterosteus aculeatus), a widely used genetic model species. The threespine stickleback fish has a re

SUBMITTER: Naftaly AS 

PROVIDER: S-EPMC8327910 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature