Hydroxycarbamide effects on DNA methylation and gene expression in myeloproliferative neoplasms.

ABSTRACT: Hydroxycarbamide (HC, hydroxyurea) is a cytoreductive drug inducing cell cycle blockade. However, emerging evidence suggests that HC plays a role in the modulation of transcription through the activity of transcription factors and DNA methylation. Examining the global mechanism of action of HC in the context of myeloproliferative neoplasms (MPNs), for which HC is the first-line treatment, will provide a better understanding of its molecular effects. To explore the effects of HC genome-wide, transcriptomic analyses were performed on two clinically relevant cell types at different stages of differentiation treated with HC in a murine MPN model. This study was replicated in MPN patients by profiling genome-wide gene expression and DNA methylation using patient blood samples collected longitud

SUBMITTER: Contreras Castillo S 

PROVIDER: S-EPMC8327916 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature