Kidney organoids generated from erythroid progenitors cells of patients with autosomal dominant polycystic kidney disease.

ABSTRACT:

Background

Kidney organoids have been broadly obtained from commercially available induced pluripotent stem cells (iPSCs); however, it has been a great challenge to efficiently produce renal organoid models from patients with autosomal dominant polycystic kidney disease (ADPKD) that recapitulate both embryogenesis and the mechanisms of cystogenesis.

Methods

Blood erythroid progenitors (EPs) from two ADPKD patients and one healthy donor (HC) was used as a comparative control to normalize the many technical steps for reprogramming EPs and for the organoids generation. EPs were reprogrammed by an episomal vector into iPSCs, which were differentiated into renal tubular organoids and then stimulated by forskolin to induce cysts formation.

Results

iPSCs derived from EPs exh

SUBMITTER: Facioli R 

PROVIDER: S-EPMC8328284 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature