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X-ray Ptychography Imaging of Human Chromosomes After Low-dose Irradiation.

ABSTRACT: Studies of the structural and functional role of chromosomes in cytogenetics have spanned more than 10 decades. In this work, we take advantage of the coherent X-rays available at the latest synchrotron sources to extract the individual masses of all 46 chromosomes of metaphase human B and T cells using hard X-ray ptychography. We have produced 'X-ray karyotypes' of both heavy metal-stained and unstained spreads to determine the gain or loss of genetic material upon low-level X-ray irradiation doses due to radiation damage. The experiments were performed at the I-13 beamline, Diamond Light Source, Didcot, UK, using the phase-sensitive X-ray ptychography method.

SUBMITTER: Bhartiya A

PROVIDER: S-EPMC8328905 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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X-ray Ptychography Imaging of Human Chromosomes After Low-dose Irradiation.

Bhartiya Archana A Batey Darren D Cipiccia Silvia S Shi Xiaowen X Rau Christoph C Botchway Stanley S Yusuf Mohammed M Robinson Ian K IK

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 20210331 1

Studies of the structural and functional role of chromosomes in cytogenetics have spanned more than 10 decades. In this work, we take advantage of the coherent X-rays available at the latest synchrotron sources to extract the individual masses of all 46 chromosomes of metaphase human B and T cells using hard X-ray ptychography. We have produced 'X-ray karyotypes' of both heavy metal-stained and unstained spreads to determine the gain or loss of genetic material upon low-level X-ray irradiation d ...[more]

PMID: 33786705

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