USP42 drives nuclear speckle mRNA splicing via directing dynamic phase separation to promote tumorigenesis.

ABSTRACT: Liquid-liquid phase separation is considered a generic approach to organize membrane-less compartments, enabling the dynamic regulation of phase-separated assemblies to be investigated and pivotal roles of protein posttranslational modifications to be demonstrated. By surveying the subcellular localizations of human deubiquitylases, USP42 was identified to form nuclear punctate structures that are associated with phase separation properties. Bioinformatic analysis demonstrated that the USP42 C-terminal sequence was intrinsically disordered, which was further experimentally confirmed to confer phase separation features. USP42 is distributed to SC35-positive nuclear speckles in a positively charged C-terminal residue- and enzymatic activity-dependent manner. Notably, USP42 directs the integr

SUBMITTER: Liu S 

PROVIDER: S-EPMC8329168 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature