Predicting the clinical outcome of oral potentially malignant disorders using transcriptomic-based molecular pathology.

ABSTRACT:

Background

This study was undertaken to develop and validate a gene expression signature that characterises oral potentially malignant disorders (OPMD) with a high risk of undergoing malignant transformation.

Methods

Patients with oral epithelial dysplasia at one hospital were selected as the 'training set' (n = 56) whilst those at another hospital were selected for the 'test set' (n = 66). RNA was extracted from formalin-fixed paraffin-embedded (FFPE) diagnostic biopsies and analysed using the NanoString nCounter platform. A targeted panel of 42 genes selected on their association with oral carcinogenesis was used to develop a prognostic gene signature. Following data normalisation, uni- and multivariable analysis, as well as prognostic modelling, were employed to develop a

SUBMITTER: Sathasivam HP 

PROVIDER: S-EPMC8329212 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature