Spatiotemporal Quantification of Cytosolic pH in Arabidopsis Pollen Tubes.

ABSTRACT: Ion-specific probes and fluorescent indicators have been key in establishing the role of ion signaling, namely calcium, protons, and anions, in plant development, providing a robust approach for monitoring spatiotemporal changes in intracellular ion dynamics. The integration of protons/pH in signaling mechanisms is especially important as reports of their biological functions continue to expand; however, attaining quantitative estimates with high spatiotemporal resolution in single cells poses a major research challenge. Here, we detail the use of the genetically encoded pH-sensitive pHluorin reporter expressed in Arabidopsis thaliana pollen tubes to assess cytosolic measurements with calibration to provide actual pH values. This technique enabled us to identify critical phenotypes

SUBMITTER: Portes MT 

PROVIDER: S-EPMC8329459 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature