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Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout.

ABSTRACT: The domestic silkworm is a type of lepidopteran insect that feeds on mulberry leaves and has high economic value because of its ability to spin cocoons. Sericin 1 is an important component of silkworm cocoons, accounting for approximately 25% of the material. In this study, CRISPR/Cas9-mediated gene editing was successfully used to destroy the sericin 1 gene, and homozygous mutants were obtained after continuous screening. Homozygous mutation resulted in premature termination of the translation of sericin 1 protein at 323 amino acids. Comparative transcriptomic and proteomic analyses of middle silk gland cells from wild-type individuals and mutants were performed on the fourth day of the fifth instar, and the results suggest that sericin 1 plays an important role in the cellular immune system. In addition, the results suggest that sericin 1 has a synergistic effect with some protease inhibitors and that the secretion of these proteins is strictly regulated. These results will provide new insights into the function and expression pattern of sericin 1 and the mechanism of silk secretion.

SUBMITTER: Ye X

PROVIDER: S-EPMC8329953 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout.

Ye Xiaogang X Zhao Shuo S Wu Meiyu M Ruan Jinghua J Tang Xiaoli X Wang Xiaoxiao X Zhong Boxiong B

FEBS open bio 20210713 8

The domestic silkworm is a type of lepidopteran insect that feeds on mulberry leaves and has high economic value because of its ability to spin cocoons. Sericin 1 is an important component of silkworm cocoons, accounting for approximately 25% of the material. In this study, CRISPR/Cas9-mediated gene editing was successfully used to destroy the sericin 1 gene, and homozygous mutants were obtained after continuous screening. Homozygous mutation resulted in premature termination of the translation ...[more]

PMID: 34185388

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Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout.

Publications

Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout.

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