Native and oxidised lipoproteins negatively regulate the serum amyloid A-induced NLRP3 inflammasome activation in human macrophages.

ABSTRACT:

Objectives

The NLRP3 inflammasome plays a key role in arterial wall inflammation. In this study, we elucidated the role of serum lipoproteins in the regulation of NLRP3 inflammasome activation by serum amyloid A (SAA) and other inflammasome activators.

Methods

The effect of lipoproteins on the NLRP3 inflammasome activation was studied in primary human macrophages and THP-1 macrophages. The effect of oxidised low-density lipoprotein (LDL) was examined in an in vivo mouse model of SAA-induced peritoneal inflammation.

Results

Native and oxidised high-density lipoproteins (HDL₃) and LDLs inhibited the interaction of SAA with TLR4. HDL₃ and LDL inhibited the secretion of interleukin (IL)-1β and tumor necrosis factor by reducing their transcript

SUBMITTER: Nurmi K 

PROVIDER: S-EPMC8329955 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature