Stage-specific transcriptomic changes in pancreatic α-cells after massive β-cell loss.

ABSTRACT:

Background

Loss of pancreatic insulin-secreting β-cells due to metabolic or autoimmune damage leads to the development of diabetes. The discovery that α-cells can be efficiently reprogrammed into insulin-secreting cells in mice and humans has opened promising avenues for innovative diabetes therapies. β-cell loss triggers spontaneous reprogramming of only 1-2% of α-cells, limiting the extent of regeneration. Most α-cells are refractory to conversion and their global transcriptomic response to severe β-cell loss as well as the mechanisms opposing their reprogramming into insulin producers are largely unknown. Here, we performed RNA-seq on FAC-sorted α-cells to characterize their global transcriptional responses at different time points after massive β-cell ablation.

Results

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SUBMITTER: Oropeza D 

PROVIDER: S-EPMC8330016 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature