Clinical application of whole transcriptome sequencing for the classification of patients with acute lymphoblastic leukemia.

ABSTRACT:

Background

Considering the clinical and genetic characteristics, acute lymphoblastic leukemia (ALL) is a rather heterogeneous hematological neoplasm for which current standard diagnostics require various analyses encompassing morphology, immunophenotyping, cytogenetics, and molecular analysis of gene fusions and mutations. Hence, it would be desirable to rely on a technique and an analytical workflow that allows the simultaneous analysis and identification of all the genetic alterations in a single approach. Moreover, based on the results with standard methods, a significant amount of patients have no established abnormalities and hence, cannot further be stratified.

Methods

We performed WTS and WGS in 279 acute lymphoblastic leukemia (ALL) patients (B-cell: n = 211; T-cell:

SUBMITTER: Walter W 

PROVIDER: S-EPMC8330044 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature