Prokaryotic single-cell RNA sequencing by in situ combinatorial indexing.

ABSTRACT: Despite longstanding appreciation of gene expression heterogeneity in isogenic bacterial populations, affordable and scalable technologies for studying single bacterial cells have been limited. Although single-cell RNA sequencing (scRNA-seq) has revolutionized studies of transcriptional heterogeneity in diverse eukaryotic systems^1-13, the application of scRNA-seq to prokaryotes has been hindered by their extremely low mRNA abundance^14-16, lack of mRNA polyadenylation and thick cell walls¹⁷. Here, we present prokaryotic expression profiling by tagging RNA in situ and sequencing (PETRI-seq)-a low-cost, high-throughput prokaryotic scRNA-seq pipeline that overcomes these technical obstacles. PETRI-seq uses in situ combinatorial indexing^11,12,18 to ba

SUBMITTER: Blattman SB 

PROVIDER: S-EPMC8330242 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature