Transcription factor-based gene therapy to treat glioblastoma through direct neuronal conversion.

ABSTRACT:

Objective

Glioblastoma (GBM) is the most prevalent and aggressive adult primary cancer in the central nervous system. Therapeutic approaches for GBM treatment are under intense investigation, including the use of emerging immunotherapies. Here, we propose an alternative approach to treat GBM through reprogramming proliferative GBM cells into non-proliferative neurons.

Methods

Retroviruses were used to target highly proliferative human GBM cells through overexpression of neural transcription factors. Immunostaining, electrophysiological recording, and bulk RNA-seq were performed to investigate the mechanisms underlying the neuronal conversion of human GBM cells. An in vivo intracranial xenograft mouse model was used to examine the neuronal conversion of human GBM cells

SUBMITTER: Wang X 

PROVIDER: S-EPMC8330525 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature