Beyond multi view deconvolution for inherently aligned fluorescence tomography.
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ABSTRACT:
SUBMITTER: Ancora D
PROVIDER: S-EPMC8333050 | biostudies-literature | 2021 Aug
REPOSITORIES: biostudies-literature
Publications
Beyond multi view deconvolution for inherently aligned fluorescence tomography.
Ancora Daniele D Valentini Gianluca G Pifferi Antonio A Bassi Andrea A
Scientific reports 20210803 1
In multi-view fluorescence microscopy, each angular acquisition needs to be aligned with care to obtain an optimal volumetric reconstruction. Here, instead, we propose a neat protocol based on auto-correlation inversion, that leads directly to the formation of inherently aligned tomographies. Our method generates sharp reconstructions, with the same accuracy reachable after sub-pixel alignment but with improved point-spread-function. The procedure can be performed simultaneously with deconvoluti ...[more]
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