Transplantable human motor networks as a neuron-directed strategy for spinal cord injury.

ABSTRACT: To repair neural circuitry following spinal cord injury (SCI), neural stem cell (NSC) transplantation has held a primary focus; however, stochastic outcomes generate challenges driven in part by NSC differentiation and tumor formation. The recent ability to generate regionally specific neurons and their support cells now allows consideration of directed therapeutic approaches with pre-differentiated and networked spinal neural cells. Here, we form encapsulated, transplantable neuronal networks of regionally matched cervical spinal motor neurons, interneurons, and oligodendrocyte progenitor cells derived through trunk-biased neuromesodermal progenitors. We direct neurite formation in alginate-based neural ribbons to generate electrically active, synaptically connected networks, characterize

SUBMITTER: Olmsted ZT 

PROVIDER: S-EPMC8333163 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature