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Reconstituting organ-level lung functions on a chip.

ABSTRACT: Here, we describe a biomimetic microsystem that reconstitutes the critical functional alveolar-capillary interface of the human lung. This bioinspired microdevice reproduces complex integrated organ-level responses to bacteria and inflammatory cytokines introduced into the alveolar space. In nanotoxicology studies, this lung mimic revealed that cyclic mechanical strain accentuates toxic and inflammatory responses of the lung to silica nanoparticles. Mechanical strain also enhances epithelial and endothelial uptake of nanoparticulates and stimulates their transport into the underlying microvascular channel. Similar effects of physiological breathing on nanoparticle absorption are observed in whole mouse lung. Mechanically active "organ-on-a-chip" microdevices that reconstitute tissue-tissue interfaces critical to organ function may therefore expand the capabilities of cell culture models and provide low-cost alternatives to animal and clinical studies for drug screening and toxicology applications.

SUBMITTER: Huh D

PROVIDER: S-EPMC8335790 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Reconstituting organ-level lung functions on a chip.

Huh Dongeun D Matthews Benjamin D BD Mammoto Akiko A Montoya-Zavala Martín M Hsin Hong Yuan HY Ingber Donald E DE

Science (New York, N.Y.) 20100601 5986

Here, we describe a biomimetic microsystem that reconstitutes the critical functional alveolar-capillary interface of the human lung. This bioinspired microdevice reproduces complex integrated organ-level responses to bacteria and inflammatory cytokines introduced into the alveolar space. In nanotoxicology studies, this lung mimic revealed that cyclic mechanical strain accentuates toxic and inflammatory responses of the lung to silica nanoparticles. Mechanical strain also enhances epithelial and ...[more]

PMID: 20576885

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A Bile Duct-on-a-Chip With Organ-Level Functions.

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| S-EPMC7048662 | biostudies-literature

Immune cell extravasation in an organ-on-chip to model lung imflammation.

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| S-EPMC10234692 | biostudies-literature

Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy.

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| S-EPMC4997336 | biostudies-literature

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| S-EPMC8869899 | biostudies-literature

Recapitulating the Vasculature Using Organ-On-Chip Technology.

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| S-EPMC7175276 | biostudies-literature

Biomimetic Human Disease Model of SARS-CoV-2-Induced Lung Injury and Immune Responses on Organ Chip System.

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| S-EPMC7646023 | biostudies-literature

Significantly enhanced lung metastasis and reduced organ NK cell functions in diet-induced obese rats.

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| S-EPMC6745596 | biostudies-literature

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| S-EPMC11661588 | biostudies-literature

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