HHLA2 deficiency inhibits non-small cell lung cancer progression and THP-1 macrophage M2 polarization.

ABSTRACT:

Background

Human endogenous retrovirus-H long terminal repeat-associating protein 2 (HHLA2) is a member of B7 family, which is upregulated in multiple tumors. However, its exact functions in non-small cell lung cancer (NSCLC) have not been fully understood. This study aimed to investigate the biological roles of HHLA2 in human NSCLC and the relevant mechanisms. In addition, the effects of tumor cell-derived HHLA2 on tumor-associated macrophage (TAM) polarization were explored.

Methods

NSCLC cell growth, migration, and invasion were assessed by colony formation and modified Boyden chamber assays. Cell cycle and the CD163+ TAMs were examined by flow cytometry. A co-culture model of THP-1 macrophages and NSCLC cells was conducted to investigate the impacts of tumor cell-derived

SUBMITTER: Sun W 

PROVIDER: S-EPMC8335813 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature