Transcriptome-wide m⁶A profiling reveals mRNA post-transcriptional modification of boar sperm during cryopreservation.

ABSTRACT:

Background

Cryopreservation induces transcriptomic and epigenetic modifications that strongly impairs sperm quality and function, and thus decrease reproductive performance. N⁶-methyladenosine (m⁶A) RNA methylation varies in response to stress and has been implicated in multiple important biological processes, including post-transcriptional fate of mRNA, metabolism, and apoptosis. This study aimed to explore whether cryopreservation induces m⁶A modification of mRNAs associated with sperm energy metabolism, cryoinjuries, and freezability.

Results

The mRNA and protein expression of m⁶A modification enzymes were significantly dysregulated in sperm after cryopreservation. Furthermore, m⁶A peaks were mainly enriched in coding

SUBMITTER: Qin Z 

PROVIDER: S-EPMC8335898 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature