Exploring functionality of the reverse β-oxidation pathway in Corynebacterium glutamicum for production of adipic acid.

ABSTRACT:

Background

Adipic acid, a six-carbon platform chemical mainly used in nylon production, can be produced via reverse β-oxidation in microbial systems. The advantages posed by Corynebacterium glutamicum as a model cell factory for implementing the pathway include: (1) availability of genetic tools, (2) excretion of succinate and acetate when the TCA cycle becomes overflown, (3) initiation of biosynthesis with succinyl-CoA and acetyl-CoA, and (4) established succinic acid production. Here, we implemented the reverse β-oxidation pathway in C. glutamicum and assessed its functionality for adipic acid biosynthesis.

Results

To obtain a non-decarboxylative condensation product of acetyl-CoA and succinyl-CoA, and to subsequently remove CoA from the condensation product, we introduced

SUBMITTER: Shin JH 

PROVIDER: S-EPMC8336102 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature