Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken.

ABSTRACT:

Background

Liquid suspension culture efficiently proliferates plant cells and can be applied to ferns because it rapidly increases the fresh weight of gametophytes. This study assessed gametophyte proliferation and sporophyte production of Pteridium aquilinum var. latiusculum using a suspension culture method.

Results

The growth curve linear phase of gametophyte cells was confirmed between 9 and 18 days of culture, and the subculture cycle was determined to be 2 weeks. A double-strength MS medium (fresh weight, 18.0 g) containing 2% sucrose and NH₄⁺:NO₃^- (120 mM, 40:80) was found to be the optimal liquid medium. Gametophytes obtained after suspension culture for 18 days did not normally form sporophytes in an ex vitro soil environm

SUBMITTER: Jang BK 

PROVIDER: S-EPMC8336368 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature