Development and optimization of a microbial co-culture system for heterologous indigo biosynthesis.

ABSTRACT:

Background

Indigo is a color molecule with a long history of being used as a textile dye. The conventional production methods are facing increasing economy, sustainability and environmental challenges. Therefore, developing a green synthesis method converting renewable feedstocks to indigo using engineered microbes is of great research and application interest. However, the efficiency of the indigo microbial biosynthesis is still low and needs to be improved by proper metabolic engineering strategies.

Results

In the present study, we adopted several metabolic engineering strategies to establish an efficient microbial biosynthesis system for converting renewable carbon substrates to indigo. First, a microbial co-culture was developed using two individually engineered E. coli

SUBMITTER: Chen T 

PROVIDER: S-EPMC8336371 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature