High-Throughput Sequencing of Small RNAs for the Sanitary Certification of Viruses in Grapevine.

ABSTRACT: Biological indexing is the method generally recognized for the certification of propagative grapevines in many countries, and it is mandatory in the European Union. It consists of the evaluation of the plant material after grafting on indicators that are inspected for symptom development. This is a lengthy process that requires well-trained workers, testing field, etc. Alternative diagnostic methods such as serology and RT-qPCR have been discarded for certification because of their intrinsic drawbacks. In turn, high-throughput sequencing (HTS) of plant RNA has been proposed as a plausible alternative to bioassay, but before it is accepted, different aspects of this process must be evaluated. We have compared the HTS of small RNAs with bioassays and other diagnostic methods from a set of 40

SUBMITTER: Velasco L 

PROVIDER: S-EPMC8336637 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature