Tissue block staining and domestic adhesive tape yield qualified integral sections of adult mouse orbits and eyeballs.

ABSTRACT: The standard histological processing procedure, which produces excellent staining of sections for most tissues, fails to yield satisfactory results in adult mouse orbits or eyeballs. Here, we show that a protocol using tissue block staining and domestic adhesive tapes resulted in qualified integral serial cryo-sections of whole orbits or eyeballs, and the fine structures were well preserved. The histological processing protocol comprises paraformaldehyde fixation, ethylenediaminetetraacetic acid decalcification, tissue block staining with hematoxylin and eosin, embedding, adhesive tape aided sectioning, and water-soluble mounting. This protocol was proved to be the best in comparison with seven other related existing histological traditional or non-traditional processing methods, according

SUBMITTER: Li Z 

PROVIDER: S-EPMC8336840 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature